第26卷 第1期 2011年1月

无 机 材 料 学 报 Vol. 26No. 1

Journal of Inorganic Materials

Jan. , 2011

DOI: 10.3724/SP.J.1077.2011.00049

文章编号: 1000-324X(2011)01-0049-06

掺锶羟基磷灰石的制备与性能研究

李志宏, 武继民, 黄姝杰, 关 静, 张西正

(军事医学科学院 卫生装备研究所, 天津 300161)

摘 要: 掺锶羟基磷灰石的骨缺损修复效果优于羟基磷灰石. 以四水硝酸钙[Ca(NO3)2·4H2O]、磷酸氢二铵硝酸锶[Sr(NO3)2]为原料, 180℃下水热处理8h制得Sr-HA. 通过XRD、FTIR、TEM、EDX和TG/DTA[(NH4)2HPO4]、

对Sr-HA的晶相、化学组成、形貌和热稳定性进行分析, 并通过细胞培养, MTT比色法对材料的细胞毒性进行评价.结果表明: Sr取代部分Ca进入HA的晶格中. 随着Sr含量的增加, Sr-HA晶粒尺寸呈减小趋势, 热稳定性下降. 掺锶对羟基磷灰石细胞毒性影响较小, 锶磷灰石具有良好的生物相容性. 关 键 词: 掺锶羟基磷灰石; 合成; 水热法; 细胞毒性

中图分类号: O613; TQ174; R783 文献标识码: A

Preparation and Property of Strontium-substituted Hydroxyapatite

LI Zhi-Hong, WU Ji-Min, HUANG Shu-Jie, GUAN Jing, ZHANG Xi-Zheng

(Institute of Medical Equipment, Academy of Military and Medical Sciences, Tianjin 300161, China)

Abstract: The biological HA in the body differs from that of pure HA in stoichiometry, composition, physical

properties and mechanical properties, because this HA is particularly prone to ion substitution. As a cation that can substitute for calcium in the structure of hydroxyapatite, strontium provokes an increasing interest because of its beneficial effect on bone formation, and prevention of bone resorption. Here, strontium-substituted HA powders were prepared by the hydrothermal method at 180℃ for 8 h using Ca(NO3)2·4H2O, (NH4)2HPO4 and Sr(NO3)2 as reagents. X-ray diffraction, Fourier transform infrared spectroscope, transmission electron microscope, energy dis-persive X-ray, and thermogravimetric-differential thermal analysis were employed to investigate the crystalline phase, chemical composition, morphology, and thermal stability of the Sr-HA. And the cytotoxicity of Sr-HA was analyzed through MTT assay. The results show that Sr is incorporated into the HA crystal structure and the crystal grain size of the Sr-HA decreases as the Sr content (under 50wt%) is increased. Moreover, the HA thermal stability decreases. However, there’s no apparent difference between the pure hydroxyapatite and all of the Sr-HA groups in cytotoxicity, which may have good biocompatibility.

Key words: strontium-substituted hydroxyapatite; synthesis; hydrothermal method; cytotoxicity

羟基磷灰石[Ca10(PO4)6(OH)2, hydroxyapatite,

HA]与人体自然骨和牙齿等硬组织中的无机质在化学成分和晶体结构上相似, 表现出优异的生物相容性和生物活性. 自然骨中HA是一种典型的缺钙羟

基磷灰石, 其准确的表述式为(Ca, M)10(PO4, Y)6(OH, X)2, 其中M为取代的阳离子, X为取代的阴离子. 取代离子可引起结晶度、溶解度的变化, 并最终引起生物学性能的变化[1]. 人工合成的HA在

收稿日期:2010-03-20; 收到修改稿日期: 2010-05-29

基金项目: 天津市自然科学基金(09JCYBJC14100); 国家自然科学基金重点项目(10832012); 军事医学科学院科研创新 重大项目(2008ZD009)

Nature Science Foundation of Tianjing (09JCYBJC14100); National Nature Science Foundation of China (10832012); Scientific Research Project of Academy of Military and Medical Sciences (2008ZD009)

作者简介: 李志宏(1977 ), 男, 硕士, 助理研究员. E-mail: lizhihong1003@tom.com 通讯联系人: 张西正, 研究员. E-mail: z56787@sohu.com